Akhil Ramani
| Akhil Ramani |
Akhil Ramani, Markus Blatz, Fusun Ozer
Fusun Ozer
Preventive and Restorative Sciences, University of Pennsylvania, School of Dental Medicine
Introduction
This in vitro study measured and compared shear bond strength (SBS) of an experimental mussel-biomimetic primer to dentin in wet surface conditions.
Methods
The occlusal surfaces of non-carious human molar teeth were ground with 180 grit silicon carbide paper to remove occlusal cusps and enamel to create flat surfaces. After removal of roots from cemento-enamel junction, the teeth were sectioned longitudinally in two perpendicular mesiodistal and buccolingual directions to prepare 4 occlusal dentin specimens from each tooth, using a low-speed diamond saw. Each dentin specimen was embedded in cylindrical molds with fast-set acrylic resin such that the flattened dentin surface was exposed parallel to the base of the mold. Dentin surfaces were polished with #600-grit silicon carbide paper to create flat dentin surfaces with standard smear layer. A total of 60 (n=20) samples were prepared and split into three study groups, the first acting as the control in dry conditions: No primer on dry surface, experimental primer on dry surface, and experimental primer on wet surface. After acid-etching and rinsing, water was micro brushed onto Group 3 only. Primer was then applied for 10 seconds to Groups 2 and 3, the surfaces for all groups were air-sprayed, micro brushed with bonding agent (Optibond Solo Plus), and light cured for 20 seconds. Cylindrical composite samples (2.1mm diameter, 3 mm height) were bonded to the dentin surfaces by packing the material into cylindrical shaped plastic matrices and curing with a light cure for 40 seconds. The specimens were stored for 48 hours at room temperature (24℃) and then mounted in a universal testing machine. SBS was determined at a crosshead speed of 0.5 mm/min and expressed in MPa. ANOVA and Pair wise comparison with Tukey tests were used for statistical analysis.
Results
Mean SBS values were 16.6 ± 6.2, 15.2 ± 4.8, and 14.9 ± 3.6 MPa for the no-primer control, dry control group, and wet experimental group, respectively. The results for the groups were not different (p>0.05). Wet surfaces did not change bonding performance of the experimental primer.
Conclusion
The pre-treatment of dentin surfaces with a mussel-biomimetic primer may improve the bond strength to the surfaces in wet surface conditions.